DETERMINATION OF ALLANTOIN LEVELS AS A RESULT OF OPTIMIZATION OF IONIC LIQUID-ULTRASOUND ASSISTED EXTRACTION OF COMFREY LEAVES BY TLC-DENSITOMETRY

Allantoin is a compound contained in comfrey leaves. This research aims to obtain optimal condition parameters of ionic liquid-ultrasound-assisted extraction (IL-UAE) leaves attract allantoin compounds. Comfrey are extracted with eight liquids by ultrasound-assisted (UAE) and screened for levels. The best liquid from the screening results optimized response surface methodology (RSM) Box-Behnken design three factors levels determine optimum conditions Determination carried out TLC densitometry. IL obtained [HMIM]Cl as evaluation optimization parameters. highest on sixth run 306.396 μg / g powder at ratio solvents powders 10 mL g, concentrations [HMIM] Cl 1 mol L time 30 minutes.